Mice were first anesthetized with a 100 mg ketamine/kg body weight (Ketaset) and 10 mg xylazine/kg body weight (Rompun) cocktail, followed by intratracheal administration of single dose of 70 μl of CLLs (Liposoma) at 48 hours before infection. Control mice were administered with PBLs (70 μl per mouse) (Liposoma).

巨噬細胞清除劑清除肺泡巨噬細胞流式驗證清除效果：

flow cytometry plots showing CLL-mediated depletion of AMs in control and Trpv1DTA mice. PBL was used for control group of mice. CLL or PBL (70 μl per mouse) was inoculated via intratracheal route at 48 hours before the CRKP infection.

品牌：Liposoma

產(chǎn)地：荷蘭

貨號：CP-005-005

規(guī)格：5ml+5ml

品名：Clodronateliposomes & Control Liposomes

代理：靶點科技

部位：肺臟

細胞：肺泡巨噬細胞（AM）

劑量：70ul

方式：滴鼻（intratracheal administration）

時間點：建模前48h

題目：Lung-innervating nociceptor sensory neurons promote pneumonic sepsis during carbapenem-resistant Klebsiella pneumoniae lung infection

期刊：SCIENCE ADVANCES

時間：6 Sep 2024

期卷：Vol 10, Issue 36

鏈接：DOI: 10.1126/sciadv.adl6162

Liposoma巨噬細胞清除劑氯膦酸二鈉脂質(zhì)體清除肺泡巨噬細胞AM：

巨噬細胞清除劑清除肺泡巨噬細胞流式驗證清除效果

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巨噬細胞清除劑清除肺泡巨噬細胞流式驗證清除效果

Depletion of AMs